| Final | |||||
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 1 | Keira Dobbs | 12 | Nova Cent'n | 40.34 |
| 2 | 2 | Laura Luthi | 12 | Co Leicester | 40.11 |
| 3 | 3 | Hayley Cliffe | 12 | Co Leicester | 39.68 |
| 4 | 4 | Isabella Hobson-Corbet | 12 | Retford | 38.50 |
| 5 | 5 | Olivia Barrett | 12 | Nova Cent'n | 38.85 |
| 6 | 6 | Betty Cresswell | 12 | DerbyPhoenix | 39.87 |
| 7 | 7 | Leah Hannaford | 12 | Radford | 40.19 |
| 8 | 8 | Leah Burgin-Rawson | 12 | Derventio | 40.34 |