| Final | |||||
| Lane | Comp.No. | Name | AaD | Club | Time |
| 1 | 1 | Ava Alexander | 13 | Leic Sharks | 39.64 |
| 2 | 2 | Evie Bevan | 13 | Co Leicester | 39.17 |
| 3 | 3 | Lily Baish | 13 | Kettering | 38.52 |
| 4 | 4 | Alice-Mae Downs | 13 | Lincoln Vulc | 37.82 |
| 5 | 5 | Nya Wilkinson | 13 | Northampton | 38.52 |
| 6 | 6 | Sophie Statham-Gill | 13 | Co Leicester | 38.56 |
| 7 | 7 | Sigourney Bolton | 13 | South Lincs | 39.37 |
| 8 | 8 | Elissia Bowen | 13 | NottmLeander | 39.69 |